first_imgThe Antarctic limpet, Nacella concinna, exhibits the classical heat shock response, with up-regulation of duplicated forms of the inducible heat shock protein 70 (HSP70) gene in response to experimental manipulation of seawater temperatures. However, this response only occurs in the laboratory at temperatures well in excess of any experienced in the field. Subsequent environmental sampling of inter-tidal animals also showed up-regulation of these genes, but at temperature thresholds much lower than those required to elicit a response in the laboratory. It was hypothesised that this was a reflection of the complexity of the stresses encountered in the inter-tidal region. Here, we describe a further series of experiments comprising both laboratory manipulation and environmental sampling of N. concinna. We investigate the expression of HSP70 gene family members (HSP70A, HSP70B, GRP78 and HSC70) in response to a further suite of environmental stressors: seasonal and experimental cold, freshwater, desiccation, chronic heat and periodic emersion. Lowered temperatures (-1.9A degrees C and -1.6A degrees C), generally produced a down-regulation of all HSP70 family members, with some up-regulation of HSC70 when emerging from the winter period and increasing sea temperatures. There was no significant response to freshwater immersion. In response to acute and chronic heat treatments plus simulated tidal cycles, the data showed a clear pattern. HSP70A showed a strong but very short-term response to heat whilst the duplicated HSP70B also showed heat to be a trigger, but had a more sustained response to complex stresses. GRP78 expression indicates that it was acting as a generalised stress response under the experimental conditions described here. HSC70 was the major chaperone invoked in response to long-term stresses of varying types. These results provide intriguing clues not only to the complexity of HSP70 gene expression in response to environmental change but also insights into the stress response of a non-model species.last_img read more

first_imgThe Badgers needed this weekend … badly.It’s hard to believe it took so long for the defending national champs to earn their first home sweep of the season and just their second series victory at the Kohl Center in their ninth attempt.It may have been Wisconsin’s breakthrough weekend of the season — with respect to that surprising road sweep of North Dakota back in December — though UW head coach Mike Eaves won’t say his team has turned a corner just yet.But even if this wasn’t the season’s highlight so far for Wisconsin (13-15-2, 10-11-1 WCHA), there were a few individuals who will look back with fond memories at the Feb. 9-10 weekend sweep of Alaska-Anchorage (11-16-3, 7-16-1).Brian Elliott has been the unluckiest player in the WCHA during the 2006-07 campaign. The conference’s preseason Player of the Year came into this series with a misleading record of 8-15-1, despite relatively decent numbers (2.32 GAA and .917 save percentage, both ranked in the NCAA Top 20).The senior netminder kept the Seawolves completely off the board for more than 110 minutes until a deflected goal got by him with nine minutes remaining in Saturday’s third period.Besides his individual effort, it made Elliott even more pleased that his team gave him some much-needed offensive support with seven goals in two games.”That’s comforting when we’re getting some goals, when you’re not facing a lot of shots, it’s tough,” said Elliott, who needed to make just 15 saves Friday for his 15th career shutout. “I think our defense played well, didn’t really make any mistakes; we just got it out and got in the offensive zone.”Eaves was glad to see his Hobey Baker candidate get some more “run support” — as the coach has called it before — and regretted the fact that Elliott hasn’t had better luck in his final collegiate year.”The only talk I’ve heard about Brian lately is that he’s having an off-year. His numbers are still darn good,” Eaves said. “It’s like that good starting pitcher in baseball that’s not getting any run support; we just haven’t been able to give Brian that goal support that would give him more W’s and fewer L’s and probably because of that, his other numbers would be better.”On the offensive side, freshman Michael Davies had scored just once since Thanksgiving weekend — one goal in 13 games — but had his breakthrough game of the season on Friday, helping the Badgers clinch the win with his team’s third and fourth goals en route to a 4-0 win.Eaves, who has been tinkering with his top two lines all season, may have found something this weekend, placing Davies on the wing with senior forwards Jake Dowell and Ross Carlson.”Mike prides himself on being an offensive guy; he’s been an offensive guy all his life. Tonight was an excellent night for Michael and his linemates,” Eaves said. “We talk about offense, [the question is] can we keep that momentum going with those young men so they can provide something that we desperately need.””We just found each other,” Davies added. “We were cycling the puck, we were moving around getting shots on goal, and that’s what we needed to do tonight and carry into tomorrow night.”Davies did carry that momentum into the next night, adding a power play assist in Saturday’s 3-1 win. That gave him three points on the weekend, his highest-scoring series this season.And just to make the weekend a little sweeter, the Badgers finally got Eaves his 100th win at Wisconsin, after failing to do so in their three previous games.”We always give a game puck out after a win to who we think played the best,” Dowell said. “Tonight, [team captain] Andrew Joudrey asked for the puck from coach, and [Joudrey] gave it to him to celebrate his 100th win. Everyone was excited.”Eaves said the milestone is best represented by the success this program has achieved over the past five years.”From where we started to where we’ve gone now and where we would like to continue to keep this program, that averages out to be 20 victories a year,” said Eaves, in his fifth year as the Badgers’ head coach. “That’s not bad. It’s a lot of work, and we need to keep continuing to push and trying to get better, have these kids improve.”Because you know what, that’s where we should be,” Eaves continued. “We should have 20-plus victories a season because this is the University of Wisconsin.”last_img read more

first_imgzoomImage Courtesy: Ole Martin Wold/Yara International Builder of specialized vessels Vard Holdings Limited has secured a contract to construct an autonomous and electric-driven container vessel for Norwegian chemical company YARA.The world’s first autonomous and electric-driven container vessel, to be named Yara Birkeland, will be delivered from Vard in Brevik in early 2020. The shipbuilder informed that the contract value is around NOK 250 million (USD 29.5 million).After delivery, the vessel will gradually move from manned operations to fully autonomous operations by 2022. It will operate in Norway, in a cargo transit between Yara’s plant in Porsgrunn to ports in Brevik and Larvik.With a length of 80 meters and a beam of 15 meters, the vessel will have a cargo capacity of 120 TEU, replacing 40,000 truck journeys a year. Yara Birkeland will reduce nitrogen oxides (NOx) and carbon dioxide (CO2) emissions.“A vessel like Yara Birkeland has never been built before, and we rely on teaming up with partners with an entrepreneurial mindset and cutting edge expertise,” Svein Tore Holsether, President and CEO of Yara, said.last_img read more